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Although there were only marginal increases in intracellular GSSG levels ( P = 0.064), IL-13 increased extracellular (apical supernatant) GSSG ( P = 0.009), while lowering both GSH ( P = 0.012) and GSH:GSSG ( P = 0.006

Sulfur dioxide prevents oxidative spoilage by scavenging hydrogen peroxide (Danilewicz 2011, Danilewicz and Wallbridge 2010) and by reacting with quinones, reducing them to the catechol form (Saucier and Waterhouse 1999) or yielding sulfonates as addition products (Nikolantonaki and Waterhouse 2012)

This is important to recognize as unnecessary transfusion of patients who are clinically well can be harmful due to iron loading

Notably, this approach identified previously reported serotonylation targets, such as GAPDH 23 and histone H3 18 , validating the robustness and specificity of our methodology (Fig