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Waters), and the mobile solutions were water (A) and acetonitrile (B) with 10 mM ammonium formatted

2015 Mar 1;170:271-80

Daneva et al., 2016)

Ascorbate peroxidase (APX) activity was assessed by monitoring the oxidation of ascorbate in the presence of H 2 O 2 [53]

Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds Irreversible - forms permanent amide bonds