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Bacteriostatic water degradation signals include visible cloudiness or particulate matter, a faint sweet or chemical odour indicating benzyl alcohol breakdown, pH drift below 4.5 or above 7.0 (testable with indicator strips), storage beyond 28 days post-puncture, or any temperature excursion above 25C for more than 6 hours

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After 15 days of culture under different treatment, the visible colonies were formed

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