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d ) HPLC analysis of intracellular levels of ZZ1 and its acidic metabolites

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Since such a sequence doesnt exist in nature, the compound is considered synthetic

Fragment of TfR on the cell membrane is secreted into the circulation by protease hydrolysis releasing soluble transferrin receptor (sTfR), which can indirectly reflect the cytosolic expression of TfR

Do, I mean, it's a pain in the ass, but I would say get a bunch of metrics, like do a blood panel workup, see where your blood sugar is, what your blood pressure is during this, all that