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These digital therapeutics have shown great success in enhancing adherence, engagement, and outcomes

All mRNA was detected by an ABI 7300 real-time PCR machine using the 2x SYBR Green qPCR Master Mix

It is generally recommended to start with less frequent application, such as every other night or every third night, and gradually increasing to nightly use as tolerated

194 Advanced imaging techniques, such as atomic force microscopy (AFM) and magnetic resonance elastography (MRE), enable researchers to visualize and quantify the nanoscale mechanical properties of muscle and ligament tissues