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Mol Syst Biol 7

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e , IB analysis of WCLs derived from HEK293 cells transfected with Flag-Smurf1

Thus, it is not surprising that there is a feedback loop where ROS activates autophagy, and activated autophagy triggers the degradation of oxidized proteins and damaged organelles to reduce ROS levels

Total protein (3040 g) was loaded in 10% and 420% acrylamide gel (Bio-Rad, Hercules, CA, USA) and transferred onto a polyvinylidene fluoride (PVDF) membrane and incubated with first antibodies in T-TBS with 1% bovine serum albumin for 12 h at 4 C