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however, because of the higher presence of intracellular proteins (cytoskeletal proteins in the T cell and CAR-T cell cocultures and mitochondrial proteins in the TCR-T cell cocultures), we applied a less stringent cutoff (10% instead of 5% FDR) (Supplementary Fig

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Our metabolic analyses showed that Gclc deficiency led to an accumulation of succinate, indicating a block in the TCA cycle

In addition, eliminating a buffer allows the method to be easily adapted for other complex samples analysis of NAC in the future