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2022 Nov;18(11):1196-1203

[DOI] [PubMed] [Google Scholar] 69.Hallberg SJ, McKenzie AL, Williams PT, Bhanpuri NH, Peters AL, Campbell WW, et al

Crystals of ShD14 were obtained by mixing 0.40 L of purified protein solution (6.9 mg mL 1 ) with 0.40 L of reservoir solution containing 100 mM CAPS (pH 10.0), 200 mM NaCl, and 20% (w/v) PEG8000 and allowing the drop to equilibrate at 20 C in 96-well Intelli-Plates (Art Robbins)

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and avoiding blanket supplementation in patients with adequate redox balance to prevent interference with physiological ROS signaling (Sies et al., 2024