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Description

& Zuhorn, I

SFN interacts with the active cysteine residues of KEAP1, dissociating KEAP1 and the subsequent activation of NRF-2 (257)

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After neutralization with 0.5 M Tris pH 6.8, fragmented RNA was purified with RNeasy Mini Kit (Qiagen), and residual DNA was removed by treatment with 0.05 U/L RQ1 RNase-Free DNase (Promega) at 37 C for 30 min, followed by neutral PCI extraction and ethanol precipitation