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A similar study examining the impact of CA on the epigenome in a general population found that tobacco consumption was an important confounding when examining the signature of CA (Marzi et al., Reference Marzi, Sugden, Arseneault, Belsky, Burrage, Corcoran and Caspi2018)

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J.Gil-IzquierdoA.MedinaS.OsorioE.Alvarez-QuinteroR.ZuluagaN.et al

Organ suspensions were subjected to serial 3-, 5- or 6-fold dilutions (depending on the state of disease progression) in modified Schneides Drosophila medium (Leitherer et al., 2017), with 12 replicates per dilution step

200 mg to 250 mg/day)