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Whiston, E

Safety of every-other-day fasting in the treatment of spinal cord injury: a randomized controlled trial

Diluted siRNA was mixed with transfection reagent and transfected into cells seeded in 24-well plates

The final pellets were suspended in Laemmli sample buffer, boiled for 5 min, and then briefly centrifuged to collect the supernatants, which were then subjected to SDS-PAGE followed by immunoblotting analysis

Toxic effects are also associated with the inhibition of key enzymatic activities, including -amylase, peptidase, malate dehydrogenase, protease, and pyruvate kinase, all of which are essential for starch hydrolysis, energy metabolism, and protein mobilization