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The tissue was pre-fixed in cold methanol 99.9% (#34860, Sigma) for 15 min and dried at 37 C for 30 sec

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When to seek medical attention: Contact your GP if you experience persistent symptoms suggestive of deficiency

Adipose tissue that is not functional also impacts the production of adipokines: the condition of leptin resistance occurs even though the leptin levels are high, while the amount of adiponectin decreases, thus the process of fatty-acid oxidation is hindered