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Dosing checklist: literature search pilot doses (e.g., 10, 50, 100 g/kg in small animal models) select dose based on safety and endpoint response document rationale

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Under normal physiological conditions, neuronal action potentials are transmitted to presynaptic terminals, causing voltage-gated Ca 2+ channels to open and extracellular Ca 2+ to flow in, prompting synaptic vesicles to release glutamate into the synaptic cleft, where glutamate binds to glutamate receptors on the postsynaptic membrane, triggering the influx of cations into the postsynaptic neuron and altering the membrane potential to achieve signal transmission

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The next day, cells were starved (2% serum) for 12 h and then treated with different compounds