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To terminate the cultures, 20 L of 0.1% Triton X-100 and 30 L of lyticase (1 mg/mL in 0.1 M phosphate buffer, pH 7.5, containing 50 mM mercaptoethanol) were added, and the plates were incubated for 1 h at 35C

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FIGURE 2 In addition, greater dietary intake of Se has been associated greater sperm concentrations in semen of men infertile men (26) and some Se supplementation studies in infertile men show improvements in testicular antioxidant activity, semen Se concentrations, sperm count, sperm morphology and motility, and fertility (13)

[DOI] [PMC free article] [PubMed] [Google Scholar] 690.Peake J.M., Della Gatta P., Suzuki K., Nieman D.C