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255 In contrast to the hepatoprotective effects of CYP1A1 and CYP3A4, the CYP4 family member CYP4A14 is upregulated in fatty livers and significantly exacerbates HFD- and MCD-induced liver lipid accumulation and NASH progression by inducing FAT/CD36 expression in the HFD- and MCD-triggered mouse NASH model

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The column was washed with lysis buffer (5 mM imodazole in lysis buffer) and increasing concentrations of NaCl (0.5 mM and 1 mM) followed by increasing concentrations of imidazole (5 mM and 20 mM, in lysis buffer) to remove contaminating proteins