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For reverse transcription, first, a 10 L primer annealing mixture (7 L of modified RNA, 1 L of a 2 M reverse primer, and 2 L of 10 mM dNTPs) was prepared and incubated to anneal the primer

"Acetyl- and propionyl-carnitine in chronic fatigue syndrome." Psychosom Med 2004;66(2):276-82

As we get older, our bodies change and process medicines differently

The total number of squares crossed by each rat (total crossing), the number of rearing (standing on two hind paws without touching the walls), and grooming were recorded