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In Vivo Inhibition Studies To further investigate the potential of TGR as a drug target, we analyzed the possible effects of AF treatment on the survival of worms in mammalian hosts

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After centrifugation, 300 L PBS was added to resuspend cells, followed by the addition of 700 L pre-cooled anhydrous ethanol drop by drop, and incubated at 4 for 1824 h