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Cells were harvested and lysed with lysis buffer (50 mM Tris, 150 mM NaCl, 10% glycerol, protease inhibitors) for 30 min

A Phase IIb trial of mRNA-4157 in combination with pembrolizumab for patients with resected high-risk melanoma showed that this combination therapy prolonged recurrence-free survival compared with pembrolizumab monotherapy and had a manageable safety profile