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The WT or mutant strains were inoculated individually into separate wells (three replicates per strain or condition) by dipping a sterile wooden round toothpick into 100 L of overnight YCFA culture in a microcentrifuge tube, then stabbing the toothpick 12 mm into the center of the soft agar, without touching the bottom of the well

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*Correspondence: Andreas Stengel [email protected] This article was submitted to Neuroendocrine Science, a section of the journal Frontiers in Neuroscience Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers