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Sample Collection The murine glioma GL261 cell line was grown in complete Dulbeccos modified Eagles medium (DMEM) containing 10% newborn calf serum, 4.5 g/L glucose, 2 mM glutamine, 100 UI/mL penicillin and 100 mg/mL streptomycin at 37 C in 5% CO 2 with media changes three times per week 16

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Interestingly, early intervention does not guarantee a full recovery

Ratiometric analysis of roGFP2-Orp1 fluorescence in its physiological state revealed mostly oxidised sensor protein, as expected and previously tested for the extracellular environment (Arnaud et al ., 2023)

Mean sera metabolites concentrations ( SD) of RA patients before (b) treatment with rituximab