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To the Raji target cells (1.0 10 4 per condition), 25% v/v freshly drawn human serum was added as source of complement in the presence of anti-CD20 IgG1 variants (0.01100 nM)

Tissue material was mechanically sliced into small pieces and washed in DMEM with 1 AntibioticAntimycotic solution (Thermo Fisher Scientific, USA)

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