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5 Clinical challenges and future perspectives 5.1 Challenges of tumor heterogeneity in the ferroptosis treatment response GBM exhibits significant molecular heterogeneity, which directly affects the efficacy of ferroptosis-targeted therapies

Cells were collected by centrifugation at 4000 r/min for 15 min and resuspended in PBS containing crystal violet (10 g/ml) and incubated at 37C at 150 r/min for 20 min

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We do not perform blood tests as the vitamins utilized in our therapies are water-soluble