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ACSF solution contains (in mM): 125 NaCl, 2.5 KCl, 1.2 NaH 2 PO 4 H 2 O, 1.2 MgCl 2 6H 2 O, 2.4 CaCl 2 2H 2 O, 26 NaHCO 3 , 11 d-Glucose

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Moreover, current tandem mass spectrometric sequencing speed and the semi-stochastic nature of data-dependent acquisition (DDA) make it challenging for phosphoproteomics to systematically and reproducibly analyze phosphorylation sites across large numbers of samples