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Indeed, inflammaging is associated with cell ROS over-production leading to oxidation/damage of cellular components, enhanced inflammation, and activation of cell death pathways

doi: 10.1038/s41598-017-16790-8 10

10.1021/acsnano.8b06400 42 LiuC.WangD.ZhangS.ChengY.YangF.XingY.et al (2019)

A review of cognitive changes during acute aerobic exercise

Tissues were lysed by transferring them to pre-filled bead mill tubes (Fisher Scientific, 15-340-154) containing lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail and homogenized using a bead mill (VWR) for 10 s