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Flotillin-1 mediates PrP C endocytosis in the cultured cells during Cu 2+ stimulation through molecular interaction

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These drugs reduce the bioavailability of copper by binding with copper, thus achieving the goal of treating cancer

For low glucose experiments cells were plated in Neurobasal-A Medium, no D-glucose, no sodium pyruvate, supplemented with 0,5 mg/ml D-Glucose, 1 mM sodium pyruvate (ThermoFisher), and the components previously described for complete NB media, and incubated at 37 and 5% CO 2 for 72 h