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To confirm these results, we knockeddown unc1 expression in a tissuespecific manner using RNAi by expressing doublestranded RNAs against the gene across the entire nervous system ( rab3 promoter) using transgenesis (Appendix Fig S8)

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Mechanistically, oxidative stress triggers endoplasmic reticulum stress through the activation of the PERK-eIF2-ATF4 and IRE1-XBP1 signaling pathways, as well as through the upregulation of CHOP (C/EBP homologous protein), which promotes apoptotic signaling (75) as shown in Figure 4

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