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Blotted membranes were incubated with 5% bovine serum albumin in PBS containing 0.05% Tween 20 for 60 min at room temperature and then incubated with primary antibodies against GSTP1 (Medical and Biological Laboratories, Nagoya, Japan), GSTA1 (Abcam, Cambridge, UK), GSTK1 (Abcam), GSTM1 (Abcam), GSTO1 (Abcam), GSTT1 (Abcam), GSTZ1 (Abcam), JNK (Cell Signaling Technology, Danvers, MA), phosphorylated JNK (R&D Systems, Minneapolis, MN), and GAPDH (Cell Signaling Technology)

was supported by 2023 Key Support Project of the Liaoning Provincial Department of Science and Technology to Support the High-Quality Development of China Medical University ([2023]61-7), by Slovenian Research Agency grant J4-60077, by the Science and Technology Planning Project of Guangdong Province (2021B1212040006), and by the Sanming Project of Medicine in Shenzhen (SZSM202411023 and SZSM202411013)

Dev Pharmacol Ther 1993

Predicting the requirement for N-acetylcysteine in paracetamol poisoning from reported dose