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Yolk sac carbohydrate levels and gene expression of key gluconeogenic and glycogenic enzymes during chick embryonic development

Tate AD, Antonelli PJ, Hannabass KR, Dirain CO

Structural analysis of -TOC biosynthesis genes and conserved motifs To characterize the functional domains, conserved domains of the 10 - TOC biosynthesis genes were analyzed using the National Center for Biotechnology Information Conserved Domain Database ( The exonintron structures of 10 - TOC biosynthesis genes were determined by comparing the coding sequences and the corresponding genomic sequences on the GSDS website ( [27]

Remington R, Bechtel C, Larsen D et al

After three cycles of freezing and thawing, the cultures were centrifuged at 1000 g for 20 min (4C) to remove cellular debris, and clarified supernatants stored at -80C