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In order to identify the optimum hirudin concentration, 2.5, 12.5, and 20 g/ml thrombin was incubated with increasing concentrations of hirudin (14 nM to 14 M) in activation buffer

In brief, 20 l of Viability/Cytotoxicity Reagent containing both GF-AFC was added to each well, followed by 30 sec mixing at room temperature and incubation at 37C for 30 min

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Traditional foods consist of vegetables, fruits, nuts, seeds, yams, mushrooms, herbal teas, meat, fish, insects, etc

Several risk factors such as aging, smoking, and oxidative stress contribute to HHcy severity [10, 11] contributing to severe pathologies, namely, neurodegenerative disorders (Alzheimers (AD) and Parkinsons disease (PD), dementia, neuropsychiatric illness), thrombosis, cerebrovascular disease, osteoporosis-associated fractures, cardiovascular disease (CVD), and cancer [12,13,14,15,16,17]