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2A, compared with VRK2-KO cells downregulating the paralog genes involved in the GSH metabolic pathway, VRK2-KO cells with GCLC, GSS, and especially SLC7A11 knockdown exhibited increased ROS level, decreased GSH level and cellular viability

Willis AJ, Indra R, Wohak LE, Sozeri O, Feser K, Mrizova I, et al

The day of imaging, 57 m thick sections were made on a cryostat (Leica CM 3050S) on Superfrost Plus Microscope Slides (Fischer Scientific, #12-550-15), incubated for 10 min in Hoechst (1:10 000 in PBS 1) for nuclei visualization, then mounted in a PBS/glycerol 50% (v/v)

Moderate to severe cases may require hospitalization, IV antibiotics, or in rare cases, surgery if theres an abscess, perforation, or recurrent severe episodes