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In this instance, and in cells where abundant endogenous biotin is expected, a blocking step with unlabeled streptavidin before applying the BHZ would reduce background noise

Table 1 displays the primer sequences of target genes using the housekeeping gene -actin as internal control along with the amplified PCR product sizes

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In conclusion, MS involves complex metabolic alterations in T cells, with enhanced glycolysis and disrupted lipid metabolism contributing to Th17/Treg imbalance, while microbial metabolites and fatty acids offer promising therapeutic avenues for immune resetting

doi: 10.1016/j.brs.2017.10.006 108