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Zhu, X., Li, B

The simultaneous reduction and derivatization reactions were stopped after 1, 5, 10, 15, 20, and 30 min by addition of 50 L of 3 mol/L PCA

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The gold nano-extract highly improved the isoenzyme pattern by eliminating the two abnormal bands and restoring the missing -EST1 and -EST3 types (Rfs 0.11 and 0.87