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Elution of specifically bound phage particles from glutathione Sepharose was carried out with glutathione buffer (20 mM glutathione, 100 mM Tris, 200 mM NaCl, 0.1% Tween 20, pH 8.0) or for Ni-NTA agarose was carried out competitively with buffer with 500 mM imidazole (500 mM imidazole, 50 mM Na 2 HPO 4 , 300 mM NaCl, 0.1% Tween 20, pH 7.5)

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After 30 min, the lysate was clarified by centrifugation (1500 g ), filtered (0.22 m filter), aliquoted and the E1E2-containing lysates were stored at 80 C

World J Gastroenterol 2014;20:11271138.ArticlePubMedPMC 23

Alencar, M.T