Free drug is rapidly removed from plasma, bound drug is released to maintain equilibrium and a concentration gradient is maintained between plasma and hepatocyte because drug is metabolised very quickly
Trafficking of DAT is tightly regulated by several posttranslational modifications (PTMs) including phosphorylation, palmitoylation, ubiquitination, and glycosylation which ultimately determine the localization of DAT to the cytoplasm or plasma membrane (Li et al., 2004
Anti-anxiety effects in rats at 0.5 g/kg (extremely low dose) produced effects within 12 minutes of intraperitoneal injection, including increased time exploring open/lighted maze areas, decreased immobile freeze reaction time, and increased exploration in open field tests
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