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Cellular analysis of the adipose tissue from HFD mice showed that GPNMB high cells, primarily associated with senescent endothelial cells, are significantly reduced due to the effect of the vaccination

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After OGD treatment, the number of Ki-67-positive endothelial cells significantly decreased, while the intracellular expression levels of senescence markers P21, P53, and the DNA damage indicator -H2AX were markedly elevated (Figure 3B)