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As pBR322 had only a moderate copy number (~30-40 copies per cell), NEB quickly adopted use of the higher copy number plasmid, pUC19, after its development by Jo Messing at the University of California Davis

By focusing on solvent exposure regions, structural optimization of compound molecules can enhance drug affinity and selectivity, improve drug solubility and bioavailability, reduce drug side effects, and overcome drug resistance

Plates were incubated overnight, and the brightest colonies were selected and grown overnight in LB with antibiotics in a 96-deep-well plate

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Curr Pharm Des 27:490504