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100 l of Ellmans reagent was added to the same tube and then the second absorbance (OD2) was measured

Subsequently, the cells were washed with PBS and stained by C11-BODIPY 581/591 (5 M) for 20 min

The regulatory effect of metformin on the microbiota extends beyond GSH to include microbial metabolites such as short-chain fatty acids (SCFAs), which have been shown to inhibit tumor proliferation and protect against intestinal injury [34,35,36]

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