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4 Discussion In this paper, we focused on tracking the associations between TRP metabolites, pteridines (neopterin, biopterin), and selected markers from the same cohort of depressive children and adolescents already published [markers of lipid profiles and oxidative stress ( The molecular mechanisms of depressive and anxiety disorders in children are not completely known ( Table 1 )

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For liquid media, we used Brucella Broth (BBL) media supplemented with 10% heat-inactivated fetal bovine serum (FBS, Life Technologies) (BB10)

However, this method is more invasive and carries a higher risk of infection