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Each experiment was conducted independently three times with duplicate measurements to ensure reproducibility and statistical robustness [1]

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Epitalons ability to reactivate telomerase has been demonstrated using the Telomeric Repeat Amplification Protocol (TRAP) assay in two key cell models: HeLa cells (telomerase-positive cancer cell line) and Human fetal lung fibroblasts (602/17)

Visualization of the bands is achieved in a second step and requires the usage of streptavadin-conjugated horseradish peroxidase (HRP) and chemiluminescent detection reagents