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Systematic substitution of each residue in the sequence with Ala enables the identification of critical amino acids governing protein bioactivity

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This may be because the addition of FeS 2 can provide more S 2 and Fe 2+ to the soil, while S 2 will form insoluble sulphide with Cd to reduce the effectiveness of Cd in the soil [30, 31], moreover, after oxidation of Fe 2+ , it will form more difficult to move oxides with Cd, which will also lead to a relative decrease in the uptake of Cd by plants [32]