Among them, the synthetic tetrapeptide AEDG (Ala-Glu-Asp-Gly, Epitalon) has been obtained from a natural peptide called epithalamin, produced in the pineal gland [13]
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doi: 10.3390/pharmaceutics14112440, 54
(15)Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- (15) Preparation of Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: (14)Fmoc-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-1050530105010603DMF3DMF2DMFFmoc-Gly-OHDIEATBTU/HOBtHBTU/HOBtBOP/HOBt105010603DMF3DMF2Fmoc-Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- In the Fmoc-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin of step (14) , add decapping reagent at 10-50C and react for 5-30 minutes, use a vacuum pump to dry up, re-add decapping reagent at 10-50C and react for 10-60 minutes, drain, wash with isopropanol for 3 times, DMF for 3 times, repeat Wash twice with steamed DMF, add the mixture of Fmoc-Gly-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt dissolved in DMF, react at 10-50C for 10-60 minutes, and dry it with a vacuum pump
