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However, what you tend to feel immediately upon injection is firmness due to swelling and inflammation (think of how a newly-bruised area will feel like a firm bump)

The more depleted your body is, the more time and consistent supplementation it will take to feel the full effects

SLC1A5 The amino acid transporter SLC1A5 (ASCT 2) is highly expressed in various tumor tissues and is associated with poor prognosis of cancer

This is more of a concern for molecular biology experiments, because human body react transcriptionally to exogenic substances which can be totally normal and benign

For this reason, an overdose is very rare and it's generally considered safe, even at high doses