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Exenatide (Byetta), approved in 2005, was based on exendin-4, a naturally occurring peptide from the Gila monster with inherent DPP-4 resistance

Within MSFraggers, precursor mass tolerance was set to +/-20ppm, fragment mass tolerance was set to +/-20ppm, tryptic cleavage specificity was applied, as well as fixed methionine oxidation and fixed N-terminal acetylation

Multiple oxidation and antioxidant systems can be activated simultaneously and run in parallel to adjust this threshold, which is associated with metabolic reprogramming of the affected cell

Fukuda K, Asoh S, Ishikawa M, Yamamoto Y, Ohsawa I, Ohta S

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