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Samples were then precipitated with 1 mL absolute MeOH at 40 C for 2 h, centrifuged (16,000 g ), and the obtained supernatants were evaporated, and the residue was resuspended and sonicated in 100 L 50 mM HEPES buffer (pH 7.4)

As a result, the low intake of dietary fiber and increased levels of fat and sugar in our food, which are typical of a westernized lifestyle and diet, may contribute to the depletion of specific bacterial taxa, at least in part [38]

However, we think behavior experiments like gait or motor behavior are more valuable in the recovery process than other evaluation methods