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doi:10.1007/s11892-019-1150-5 78

doi: 10.1016/j.mce.2020.111085 21 MaQBealJRBhurkeAKannanAYuJTaylorRNet al

Performance characteristics Synonyms ALT Sample type Serum, plasma, animal tissue Sensitivity 0.01 U/L Detection range 0.01-0.83 U/L Detection method Fluorescence method Assay type Enzyme Activity Assay time 75 min Precision Average inter-assay CV: 9.800000000000001%Average intra-assay CV: 2.3% Other instruments required Pipettor, Vortex mixer, Centrifuge Storage -20 C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-0.83 U/L)

Less common: Temporary skin discoloration at the injection site

The most commonly cited preclinical protocols report 10 g/kg in published rat studies of tendon/ligament healing