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Brains were placed into ice-cold dissection media containing 50% modified essential media (MEM), 50% Hanks' buffered salt solution (HBSS), 2 mM glutamine (GlutaMAX-I), 1% antibiotic-antimycotic, 26.6 mM HEPES, 10 mM glucose, and 5 mM MgSO 4

The prevalence of TBI associated injuries that result in neuronal loss and decreased synaptic connectivity denote the need for therapies which support neuronal repair and/or replacement