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The cell pellet was resuspended in a 37% Percoll solution and centrifuged at 1200 g for 30 min to remove myelin debris

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Neuronal apoptosis and DNA damage markers (Caspase-3, H2A.X, 8-OHdG) were significantly reduced by ZO treatment

Potential of co-culture of nucleus pulposus mesenchymal stem cells and nucleus pulposus cells in hyperosmotic microenvironment for intervertebral disc regeneration