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OTUB1, GCLC, and GS antibodies were obtained from Abcam, while beta-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase, HA, and Ub antibodies were obtained from Cell Signaling Technology

Therefore, regulation of ferroptosis in cardiomyocyte and attenuation of myocardial inflammation in DCM by Nrf2 may provide new therapeutic options for DCM