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Morito N, Yoh K, Itoh K, Hirayama A, Koyama A, Yamamoto M et al

If you are over, push a tiny bit back into the vial

Then try the escalation again

Class II Medications: Beta Blockade Beta-blockers are central to managing both supraventricular (e.g., APB, ST, SVT, AF, atrial flutter) and ventricular arrhythmias by blocking beta-adrenergic receptors, reducing sympathetic stimulation, slowing sinus and AV nodal conduction, and decreasing automaticity (6)(11)(35)

For experiments in 384-well plates, wells were aspirated, rinsed with PBS and incubated with a fixative and staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399)