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Despite this, the interaction between the Zn atom and the oxygen atom in GHB remains relatively strong

doi: 10.1016/j.semnephrol.2018.01.001

Collected cells were resuspended into Cellartis DEF-CS medium supplemented with GF-1, GF-2, and GF-3 and seeded onto a 6-well tissue culture plate pre-coated with LDEV-free, hESC-qualified Matrigel (Corning Inc.) at a cell density of 2.1x105 cells/cm2